Introduction
Brief History
Goals of this Research
1) Answer the following questions:What are the key elements to creating domoic acid?
Which Bacteria are responsible for this?
Is this a product of chemosynthesis or photosynthesis?
Or a fatty acid being produced, or some other vital chemical for the mutual relationship between the bacteria and the diatom?
Can domoic acid be found in the archeological record? Did domoic acid exist 250 years ago?
Chapter 1
Research Papers
Mary Silver Toxic diatoms and domoic acid in natural and iron enriched waters of the oceanic Pacifichttp://domoicacid.pbworks.com/w/page/36647866/Research-Papers
Chapter 2
I reseached Mary Silver's paper Toxic diatoms and domoic acid in natural and iron enriched waters of the oceanic Pacific, I found the following statement. "" I am intrigued by this statement in that it speaks to the potential source elements for the chemical reaction that is required to H2O+NaCl+?+?+?=C22H16NO6Ultimately we need to get it down to what we do and don't know for example
So again while this intruiges me, the statement its self is merely a general observation of conventional wisdom. Where do we turn to if we want someone the analyze the chemical reactions that are taking place? How many research projects do we need before someone asks the right question. And determines the source elements. When I say source elements I mean to say reactants in the chemical process that produces domoic acid.
Chapter 3: Identification and enumeration of cultured and wild Pseudo-nitz- schia (Bacillariophyceae)
http://www.cencoos.org/sections/news/MontBay_HAB_experiment_2010.shtml
These guys are tracking the Pseudo-nitzschia in real time, and publishing within four months.
Lots of great field research, some very cool technology and toys. Unfortunately, they don't appear to have any relevant data for my thesis criteria. Hopefully, they will find something during the synthesis period!
Back in 2007:
"It's not a single, uniform event--the blooms are spotty, both in time and in locations throughout the bay," said Mary Silver, professor of ocean sciences at UCSC. "When you look at satellite images, the blooms are almost like weather systems or cloud formations--they come and go on a day-to-day basis. But the animals are being affected."
ORIGINAL ARTICLE
Direct contact between Pseudo-nitzschia multiseries and bacteria is necessary for the diatom to produce a high level of domoic acid
Abstract
Pseudo-nitzschia multiseries loses most of its ability to produce domoic acid when it is cultured axenically. However, domoic acid production recovers when bacteria from the original culture are reintroduced to axenic cultures, indicating a bacterial association with domoic acid production in this species. In order to determine the role of bacteria in domoic acid production, an axenic strain prepared from a nonaxenic strain of P. multiseries was inoculated into media in cellophane tubes, which were then placed in a bottle containing the original nonaxenic culture. Both strains showed characteristic domoic acid production similar to that previously observed. Domoic acid level in the cells of the axenic strain in the tubes was much smaller than that of the cells outside the tubes. These results show that direct contact between living bacteria and P. multiseries is necessary for producing high levels of domoic acid inP. multiseries.
For the latest Red Tide Forcast:
http://tidesandcurrents.noaa.gov/hab/
For the latest Red Tide Forcast:
http://tidesandcurrents.noaa.gov/hab/
Keywords Co-culture of axenic and nonaxenic strains - Domoic acid - Pseudo-nitzschia multiseries
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